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Ambion: May 20, 2005

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Ambion has recently introduced the TRI reagent.

According to the company, the reagent is used for the isolation of total RNA, or the simultaneous isolation of RNA, DNA, and protein, from a variety of biological samples.

The reagent "combines phenol and guanidine thiocyanate in a monophase solution to facilitate the immediate inhibition of RNase activity," Ambion said. "Biological samples are homogenized or lysed in TRI Reagent, [and] the subsequent addition of bromochloropropane or chloroform results in the separation of the homogenate into aqueous and organic phases.

"RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase," the company added. "The RNA can then be precipitated from the aqueous phase with the addition of isopropanol. The entire protocol can be completed in approximately 1 hour."

Additional details about the reagent can be found at http://www.ambion.com/catalog/CatNum.php?9738.

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