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Agencourt Bioscience s RNAClean, NuGen Technologies Ovation Biotin, Panomics TranSilent TF, BD Biosciences Clontech s Knockout Clone & Confirm, Ambion

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Agencourt Bioscience said this week that it has released RNAClean, a reagent system for the purification of nucleic acids from a number of enzymatic reactions including antisense RNA and RNA labeling reactions.

According to the company, RNAClean can be automated for use in 96- or 384-well formats.

Additional details about RNAClean can be found at http://www.agencourt.com/products/spri_reagents/rnaclean/.

Agencourt also said that it is introducing a new tube-based magnetic separation device called SPRIstand, which is designed to complement the company’s suite of plate-based separation devices.


NuGen Technologies said this week that it has launched the Ovation Biotin system for RNA amplification and labeling.

The system, said the company, is optimized for use with Affymetrix’s GeneChip arrays, and allows for expression analysis with as little as five nanograms of RNA starting material.

Details about the Ovation Biotin system can be found at http://www.nugentechnologies.com/products/biotin.shtml.


Panomics recently introduced its TranSilent TF siRNA vectors.

According to the company, the vectors consist of a mix of up to three different plasmids that target the mRNA of 40 specific human transcription factor genes.

The vectors, which have been functionally tested by western blot analysis, encode RNA sequences that are post-transcriptionally convered into siRNA-like transcripts, said Panomics.

Additional details about the vectors, including ordering information, can be found at http://www.panomics.com/SR1000.html.


BD Biosciences Clontech recently introduced its Knockout Clone & Confirm PCR kits.

The company said that the kits allow researchers to generate candidate shRNA expression cassettes precloned into vectors in as little as 4.5 hours.

The cassettes, said BD Biosciences Clontech, can also be engineered to co-express a flourescent marker.

Details on the kits, as well as ordering information, can be found at http://www.bdbiosciences.com/clontech/archive/JAN04UPD/knock out.shtml.


Ambion has added to its corporate web site a recent presentation by David Dorris, the company’s business development manager.

The presention is entitled “Predesigned and Validated siRNAs and Their Use in Functional Assays,” and can be found at http://www.ambion.com/techlib/presentations/siRNA/.

The Scan

Pig Organ Transplants Considered

The Wall Street Journal reports that the US Food and Drug Administration may soon allow clinical trials that involve transplanting pig organs into humans.

'Poo-Bank' Proposal

Harvard Medical School researchers suggest people should bank stool samples when they are young to transplant when they later develop age-related diseases.

Spurred to Develop Again

New Scientist reports that researchers may have uncovered why about 60 percent of in vitro fertilization embryos stop developing.

Science Papers Examine Breast Milk Cell Populations, Cerebral Cortex Cellular Diversity, Micronesia Population History

In Science this week: unique cell populations found within breast milk, 100 transcriptionally distinct cell populations uncovered in the cerebral cortex, and more.