This webinar illustrates how single-cell methylation sequencing can be applied to gain significant insight into epigenetic heterogeneity in disease states, advancing cancer research discoveries.
Our speaker, Jennifer Flournoy of Perelman School of Medicine at the University of Pennsylvania, discusses a single-cell methylation approach from Swift Bioscience that her team is using to study pediatric leukemia and gliomas.
Swift's Accel-NGS Adaptase Module enables construction of next-generation sequencing libraries from bisulfite-converted, single-stranded DNA from single cells. This module is optimized to maximize the recovery of DNA containing uracil residues and low concentrations of AT-rich template. The resultant libraries consistently exhibit superior complexity with reduced composition bias to provide a more faithful representation of the methylome. This approach has demonstrated greater than 2-fold increase in read mapping rate as compared to other methods, significantly improving the data output per run while reducing the sample sequencing cost.
In this webinar, Jennifer Flournoy and her colleague Dr. Hao Wu details how they are using this single-cell methylation method to contribute to the generation of multi-omic human tumor atlases and to inform therapeutic approaches for pediatric cancers.