This webinar will discuss a study that combined single-cell gene expression and spatial gene expression to understand the evolution of sepsis in the kidney at the cellular and molecular level.
Understanding the pathophysiology of acute kidney injury is crucial for the comprehensive treatment of sepsis and its complications, but the exact cellular and molecular contributors to this multifaceted response remain unknown. Furthermore, the kidney is a highly complex organ, in which epithelial, endothelial, immune, and stromal cells are at constant interplay.
In this webinar, Dr. Takashi Hato of Indiana University will describe a project that used both Chromium single-cell gene expression and Visium spatial gene expression solutions to provide a detailed and precise view of the evolution of sepsis in the kidney, as well as identify potential SARS-CoV-2 entry points.
Dr. Takashi Hato will discuss details of the study and its findings, including:
- Comprehensive spatial and temporal transcriptomic profiling of the kidney in a murine sepsis model, extending from early injury well into the recovery phase
- Identification and localization of known and novel renal cell populations in the kidney microenvironment, including regional expression of essential components of the SARS-CoV-2 entry mechanism
- Key changes identified in gene expression that altered cellular functions and explain features of clinical sepsis
- How this rigorous spatial and temporal definition of murine sepsis will uncover precise biomarkers and targets that can help stage and treat human sepsis