Reduced representation next-generation sequencing (NGS) methods are driving understanding of both physical and structural variation in all species. But these services are not created equal. The diversity of species means that not all breeding programs can rely on well-defined reference genomes. For many species, customized enzymes and expertise help uncover more variations and make more informative decisions on breeding selections.
For example, genotyping by sequencing (GBS) is a useful approach for species where there is little or no previous genetic information available. The approach provides a means for SNP discovery for lesser interrogated species and unique populations where whole-genome sequencing would not be financially feasible due to complexities and larger genome sizes.
In this panel discussion, three experts will talk through applications of targeted genotyping by sequencing, specifically focusing on how customized enzymes coupled with expertise from LGC, Biosearch Technologies, improved their breeding programs.