Methyl-Seq Studies in Rodents and Humans Identify Stress-Related Epigenomic Changes | GenomeWeb
June 02, 2016
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Agilent Technologies

Methyl-Seq Studies in Rodents and Humans Identify Stress-Related Epigenomic Changes


Assistant Professor, Department of Psychiatry and Behavioral Sciences, Johns Hopkins University

This webinar discussed the use of the Methyl-Seq platform in mouse, rat, and human studies to demonstrate how chronic exposure to stress and glucocorticoids can modify the epigenome in ways that may be relevant to disease.

During this webinar, Dr. Richard Lee, assistant professor in the Department of Psychiatry and Behavioral Sciences at Johns Hopkins University, discussed a series of studies in rodents and humans intended to elucidate the epigenomic changes linked to stress.

Dr. Lee and colleagues first sought to identify epigenomic targets of blood and brain in mice treated with glucocorticoids using the mouse SureSelect Methyl-Seq Target Enrichment platform and bisulfite pyrosequencing. They then designed and implemented a rat version of the Methyl-Seq to identify DNA methylation changes associated with stress in adolescent rats exposed to chronic variable stress for three weeks.

Dr. Lee outlined the details of these studies and their findings, which indicate that both glucocorticoids and stress can modify the epigenome.

For Research Use Only. Not for Use in Diagnostic Procedures.

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