This webinar discusses a mass spectrometry-based metaproteomic approach to examine microbiome temporal functional shifts during microbial colonization of the preterm human infant gut.
Robert L. Hettich of Oak Ridge National Laboratory will discuss his recent work developing advanced “shotgun” mass spectrometry techniques for the comprehensive characterization of microbial metaproteomes. His lab's recent work has focused on improved methods for cellular lysis and proteome extraction, coupled with automated multi-dimensional LC-MS/MS on QExactive mass spectrometric systems. This has been coupled with an improved data analysis/data mining pipeline for enhancing peptide/protein identification and quantification.
Dr. Hettich will discuss a study in which fecal metaproteomes of 94 preterm infant samples, each collected at discrete time points over several months, were measured by LC-MS/MS and yielded identification of tens of thousands of proteins, many of which corresponded to enzymes that mapped onto a large and deeply interconnected network of metabolic reactions.
Tracking metabolic function rather than discrete proteins has the potential of revealing how microbial metabolic function progresses across development of the preterm human infant gut microbiome, and how the overall metabolic potential of the system expands or contracts temporally.
The time-course metaproteomics measurements revealed core metabolic pathways in both human and microbial proteins, indicating the establishment of the mutualistic relationship between the microbiome and human host early in infancy. In total, these results reveal functional stability and inter-individual signatures of the preterm infant gut microbiome.
This webinar is part of the 2020 GenomeWeb/Association of Biomolecular Resource Facilities webinar series, which highlights methods, techniques, and instrumentation that support life science research.