This webinar discusses a target enrichment workflow for high-confidence detection of variants. Our speaker, Rajesh Singh of MD Anderson Cancer Center, details a project that used this workflow for variant detection in chronic lymphocytic leukemia gene targets.

Target capture and enrichment of genomic regions of interest is a crucial but challenging step for successful targeted next generation sequencing. Challenges include compatibility with DNA quality and input, uniform and efficient capture of the targeted genomic regions, and high-confidence detection of a variety of variants.

The HaloPlex^HS target enrichment workflow uses circularization probes for rapid capture and enrichment of genomic areas of interest with high specificity for NGS library preparation. The use of endonuclease digestion of the template and molecular barcodes provide overlapping amplicons for enhanced sequencing coverage and high-confidence detection of variants.

In this webinar, Dr. Singh discusses how his team applied the HaloPlex^HS workflow to variant detection in CLL gene targets. 

For Research Use Only. Not for use in diagnostic procedures.