GenomeWeb/ABRF 2016 Webinar Series: The ABRF NGS Study, Phase 2: DNA Sequencing Platforms | GenomeWeb
November 15, 2016
Sponsored by
New England BioLabs

GenomeWeb/ABRF 2016 Webinar Series: The ABRF NGS Study, Phase 2: DNA Sequencing Platforms

GenomeWebinar

Co Founder, MSK/dx LLC

Associate Professor, Weill Cornell Medical College

Chair ABRF Metagenomics Research Group and Extreme Microbiome Project,
Manager, Massively Parallel Sequencing Facility,
University of Vermont Cancer Center

This webinar provides an update on Phase 2 of the ongoing ABRF Next Generation Sequencing Study, an effort to evaluate the performance of NGS platforms and to identify optimal methods and best practices. Phase 1 of the study focused on RNA sequencing, while Phase 2 is focusing on genomic DNA samples.

In particular, Phase 2 of the ABRF NGS study aims to address three questions applicable to most technologies being used for deep sequencing of genomic DNA: 1) for a typical combination of sample preparation method and sequencing instrument (a “platform”), what levels of intra- and inter-laboratory variation should be expected; 2) how is a platform affected by DNA exposed to formalin fixation ; and 3) how is a platform affected by DNA that contains a skewed nucleotide composition?

Sequencing for the study is being performed by independent academic service laboratories not affiliated with reagent or instrument vendors. The use of well-characterized, publicly available reference samples and uniform protocols within each platform will generate baseline data sets against which alternative methods, hardware upgrades, and any sequencing lab’s performance can be compared.

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