In this webinar, Michael Quail of the R&D Sequencing team at the Wellcome Trust Sanger Institute will provide an expert perspective on library prep for next-generation sequencing.
The Sanger Institute is a large-scale biomedical research and genome center funded by the Wellcome Trust. The institute was an early adopter of Illumina sequencing technology and was one of the first labs in the world to automate library prep.
Dr. Quail discusses problems and biases that can occur during NGS library prep and ways to avoid them. He reviews the various steps in sequencing library construction and discuss how biases in genomic content and sample representation can arise in sequencing libraries.
Dr. Quail's presentation includes recommendations on how to avoid such biases, resulting in more even sequence coverage and fewer samples with insufficient coverage for meaningful analysis.