Improved nucleic acid quantitation from precious samples is a key driver for the concept of liquid biopsy. By quantifying miRNA from serum/plasma samples as well as biofluids, such as cerebral spinal fluid, key biomarkers can be detected without the need for invasive tissue-based biopsy.
In this online seminar, Jo Vandesompele of the Center for Medical Genetics at Ghent University will discuss methods for improving the analysis of microRNA expression from a range of samples.
Dr. Vandesompele was a co-author of the microRNA quality control (miRQC) study, published in 2014. In this study, investigators analyzed the sensitivity, reproducibility and dynamic range of multiple technologies, including small RNA sequencing, reverse transcription–quantitative PCR, and microarray hybridization. The researchers determined that qPCR was the gold standard for the quantification of miRNA.
Dr. Vandesompele's presentation will provide an overview of the use of preamplification and minimum sample inputs to maximize the dynamic range of miRNA expression studies.