In this two-part webinar, Dr. Elin Gray, from Edith Cowan University, and Ms. Weiwei Zhao, from Kingmed diagnostic, will compare the highly sensitive, multiplexed UltraSEEK technology, on the MassARRAY system, to digital droplet PCR (ddPCR) results on melanoma and non-small cell lung carcinoma samples.
Dr. Elin Gray will first demonstrate how the UltraSEEK Melanoma panel, probing for 61 mutations over 13 genes, provided equivalent analytical sensitivity and accuracy to ddPCR. This study included a blinded analysis of 68 mutations detected in 48 plasma samples from stage IV melanoma patients. Ms. Weiwei Zhao will also discuss her study between UltraSEEK EGFR to ddPCR for the detection of T790M, and other EGFR activating mutations in NSCLC. In this study, 94 cfDNA samples from advanced lung cancer patients, who were progressing on EGFR TKI, were run on UltraSEEK and ddPCR. Both UltraSEEK EGFR and ddPCR were able to achieve 0.1% minor allele fractions.
The two studies summarized in this webinar further demonstrate the benefits of using a highly sensitive and multiplexed technology when testing plasma-derived ctDNA for somatic mutations in cancer.