This webinar will discuss SCoPE2 (Single Cell Proteomics by Mass Spectrometry, Version 2), an approach for quantitatively analyzing proteins in single cells using only commercially available equipment and reagents.
Harrison Specht of Northeastern University will share details of the methodology, which lowers cost and hands-on time by introducing automated and miniaturized sample preparation while substantially increasing quantitative accuracy.
Using SCoPE2, Specht and colleagues quantified more than 2,700 proteins in 1,018 single monocytes and macrophages in 10 days of instrument time, and the quantified proteins allowed them to discern single cells by cell type. Parallel measurements of transcripts by 10x Genomics scRNA-seq suggest that SCoPE2 samples 20-fold more copies per gene, thus supporting quantification with improved count statistics. Joint analysis of the data indicated that most genes had similar responses at the protein and RNA levels, though the responses of hundreds of genes differed.
In his presentation, Specht will share details on how researchers can adopt SCoPE2 in their labs. He will discuss equipment that can be used to successfully execute SCoPE2 sample preparation, as well as reagent selection and study design, including the types of cells that are likely to perform well with SCoPE2. Additionally, he will discuss approaches to optimizing liquid chromatography and mass spectrometry instrumentation for SCoPE2 samples.
