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December 10, 2020
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Accelerating Forward Engineering and Genome Discovery with CRISPR: E. Coli Case Studies


Director, Analytical Biochemistry Applications Development, Inscripta

Senior Scientist, Applications Development, Inscripta

Head of Applications Enablement, Tecan

CRISPR has developed into a core tool to facilitate genome editing, inspiring transformative ideas in engineering biology. However, first-generation CRISPR technologies have been limited in scalability, accessibility, edit variety, and ease of use, restricting the potential of the technology.

Inscripta’s Onyx platform addresses these limitations by combining easy-to-use software with an automated benchtop device, enabling high efficiency, massively parallel, precision-engineered edits to Saccharomyces cerevisiae and Escherichia coli genomes. The platform offers an end-to-end solution from design through engineered strain library and analytics, accelerating the Design, Generate, Test and Learn cycle.

This webinar will present two applications in E. coli that leverage this platform technology. One application uses forward engineering with libraries totaling more than 200,000 different designs to increase lysine production by 14,000-fold. The second application is a targeted library approach with more than 900 edits, delivering new biochemical and structural insights underpinning resistance to otherwise growth-inhibitory compounds.

These applications demonstrate the power of genome-wide editing for rapid strain development.

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