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May 10, 2019

IDT Custom NGS Adapters, Lotus DNA Library Prep Kit

Integrated DNA Technologies has launched custom NGS adapters, which customers can order in three configurations using the company's Custom Adapter Configuration online ecommerce tool: TruSeq-compatible full-length adapters, TruSeq-compatible stubby adapters and indexing primers, and Nextera-compatible indexing primers.

In addition, IDT has released the Lotus DNA Library Prep kit, an enzymatic library preparation solution for whole-genome sequencing and hybridization capture applications. The kit is optimized for use with IDT's custom adapters and xGen capture products and has a 2-hour workflow. It is compatible with TA-ligation adaptors and can be tailored to different applications when combined with the new adapters.

May 06, 2019

The Sequencing Center Human WGS Service

The Sequencing Center of Fort Collins, Colorado has launched a human whole-genome sequencing service for medical research. It includes data analysis as well as data management services. Customers can also request DNA extraction to be performed at no additional cost. According to the firm's website, sequencing projects take 10 to 15 business days to complete.

May 01, 2019

Arc Bio Galileo Pathogen Solution, GPS-Transplant

Arc Bio has launched the Galileo Pathogen Solution product line and first product, GPS-Transplant, a research-use-only next-generation sequencing test to aid in monitoring post-transplant patients for viral infections. GPS-Transplant detects the most common viral infections among transplant patients, including cytomegalovirus, Epstein–Barr virus, BK virus, and human adenovirus. The test also detects different variances and genotypes of the viruses, as well as more rare infections. GPS-Transplant also offers the ability to detect antiviral resistance mutations, and performs simultaneous quantitative detection of more than 350 viral strains. It is a sample-to-report solution built around the Illumina NGS platform that consists of reagents, built-in assay controls, and Galileo Analytics, a cloud-based proprietary software that provides quick and reliable results, an intuitive user interface, and detailed, actionable reports. The analytics software leverages Arc Bio's curated pathogen database called ArcIve, and also incorporates AMR resistance prediction technology which Arc Bio launched last year.

Apr 30, 2019

Thermo Fisher Scientific MagMax Nucleic Acid Isolation Kits

Thermo Fisher Scientific has launched several new MagMax nucleic acid isolation kits. The kits, which uses MagMax magnetic bead technology, are optimized for Thermo Fisher KingFisher Flex and Duo Prime instruments. The MagMax Microbiome Ultra Nucleic Acid Isolation Kit is designed to isolate RNA and DNA from microbiome samples such as stool and soil samples; the MagMax Viral/Pathogen Ultra Nucleic Acid Isolation Kit is made for recovering RNA and DNA from difficult-to-lyse microorganisms, including gram-positive bacteria, yeast, and fungi; and the MagMax Viral/Pathogen Nucleic Acid Isolation Kit is for isolating RNA and DNA from viral particles and gram-negative bacteria found in samples such as blood, swabs, urine, and viral transport media.

Apr 30, 2019

New England Biolabs NEBNext Enzymatic Methyl-seq

New England Biolabs has launched NEBNext Enzymatic Methyl-seq (EM-seq), an enzyme-based method for methylation sequencing that is an alternative to bisulfite sequencing. The EM-seq kit involves a two-step enzymatic conversion that first protects 5-methyl cytosine and 5-hydroxymethyl cytosine from deamination using TET2 and an oxidation enhancer, and then deaminates unmethylated cytosines, converting them to uracil. Because the process does not rely on chemical treatment, as with bisulfite conversion, the DNA is not damaged, according to the company. The kit includes the required reagents for EM-seq conversion and library preparation for sequencing on Illumina instruments.

Apr 24, 2019

Human Diagnostics Worldwide, Eiken Chemical, FIND Malaria-LAMP

Human Diagnostics Worldwide, Eiken Chemical, and the Foundation for Innovative New Diagnostics launched Malaria-LAMP for the detection of malaria caused by Plasmodium vivax parasites. The group said that it is the first commercially available molecular test for P. vivax-associated malaria. The assay uses loop-mediated isothermal amplification developed by Eiken and is exclusively distributed by Human Diagnostics. FIND and the Hospital for Tropical Diseases evaluated the prototype test and coordinated in-country clinical performance studies in Columbia and Peru. The test can differentiate between Plasmodium pan species, P. falciparum, and P. vivax, and has a sensitivity of 84 percent to 94 percent and a specificity of greater than 92 percent. According to the group, the assay's limit of detection is one to two parasites per microliter.

Apr 24, 2019

Pacific Biosciences Sequel II System

Pacific Biosciences has launched it Sequel II single-molecule sequencing instrument. The new system runs SMRT chips that contain 8 million wells, or zero-mode waveguides (ZMWs). The system delivers around eight times greater data output than the firm's original Sequel instrument, which had 1 million ZMWs. According to the company, its HiFi reads, which make use of PacBio's circular consensus sequencing (CCS) protocol, have greater than 99.9 percent accuracy. As of last month, five early access customers had run 58 SMRT cells, obtaining average yields per SMRT cell for the CCS protocol of 250.4 gigabases and average per-cell yields for its continuous long read protocol of 67.4 gigabases.

Apr 23, 2019

Cell Microsystems AIR Software Update, HexaQuad Array

Cell Microsystems has released new software for its AIR system, which automates cloning workflows using brightfield imaging to eliminate the need for fluorescent markers for sorting and confluency measurement. In addition, another new feature automates cell-sorting based on fluorescent signal intensities to characterize complex phenotypes.

Cell Microsystems has also launched the HexaQuad array, which it has designed to simultaneously run 24 distinct experiments on a single consumable. The firm said that the array is suited for labs who perform high-throughput CRISPR editing in single guide or guide pools, which allows for scaling of hundreds of clones per week and reducing a two-month workflow. Combined with the new software, the array provides a five-day workflow for researchers.

Apr 22, 2019

MycoDART MycoDART-PCR

MycoDART has launched MycoDART-PCR, a dual amplification real-time PCR DNA test for the rapid detection of Candida aurus. Pending approval by the US Food and Drug Administration, the test can be ordered currently only through RealTime Laboratories and only with a doctor's order. The launch comes amid reports that C. auris, which is highly resistant to antifungal medications, is spreading throughout the US. C. auris is 1 of 6 Candida species that MycoDART-PCR can detect. It offers a specificity and sensitivity of 95 percent and has been validated for blood, body fluids, and tissue MycoDART said. It can also be run to detect for the presence of C. auris on environmental sufraces. For such purposes, a doctor's order is not needed for the test. 

Pages

New research shows that scientists need to do a better job of including a wider diversity of African genomes in their analyses, STAT says.

A new paper in Science shows that men are still winning a large majority of the most sought-after NIH grants.

Nature Research and the Estée Lauder Companies are awarding efforts to encourage girls to pursue careers in the STEM fields.

In Science this week: beneficial genetic variants inherited from archaic Neanderthal and Denisovan hominins, and more.