Sygnis TruePrime RCA Kit

Sygnis has launched the TruePrime RCA kit for the direct amplification of circular DNA targets. Combining Sygnis' proprietary DNA primase TthPrimPol with its Phi29 polymerase, the TruePrime technology does not need synthetic random primers to start the reaction. The TruePrime RCA kit enables direct amplification of plasmids from purified DNA or bacteria, reducing time involved in this standard process from 18 hours to 1 hour. Additionally, the kit can be used to amplify large circular vectors such as BACs and cosmids needed for genetic manipulation, Sygnis said.