SeqWell PlexWell Single-Cell Rapid Kit

SeqWell has launched its PlexWell Single-Cell Rapid Kit for the preparation of next-generations sequencing libraries for single-cell RNA sequencing. The kit improves upon the Smart-seq2 method by combining cDNA synthesis and amplification, and reducing the QC and normalization burden by 75 percent to achieve a single-day library prep workflow. This simplified approach can produce sequencing-ready, full-length transcriptome libraries from hundreds to thousands of sorted cells at a time, the company said. Other features include iterative barcoding and auto-normalization to create balanced library pools without the need for further sample or library normalization. The method has been optimized for low cDNA inputs to maintain library complexity, improve detection of low-abundance targets, and preserve variant and isoform information, the company said.