Rubicon Genomics has launched its ThruPLEX Plasma-seq kit for the preparation of DNA libraries to analyze cell-free DNA found in plasma and other biofluids. The new kit is designed for Illumina next-generation sequencing platforms and has been evaluated by researchers at the Karolinska Institute and Cancer Research UK.
ThruPLEX Plasma-seq has a simple and fast workflow, requiring only three steps in a single tube and two hours of time, with no purification or sample transfers, and yields highly reproducible NGS libraries from less than 1 nanogram and up to 30 nanograms of cell-free DNA in plasma. The optimized repair and ligation chemistries and a modified protocol provide higher library diversity at low input amounts and broad and consistent GC coverage, Rubicon said. The kit is intended for use with plasma samples for applications including analysis of circulating tumor DNA using whole-genome, whole-exome, and targeted gene sequencing, as well as for non-invasive prenatal testing.