Promega has launched its Wizard HMW DNA Extraction Kit, a tool for researchers working with large fragments of genomic DNA. According to the company, the new HMW DNA product, which joins the firm's existing portfolio of both manual and automated kits to extract a variety of nucleic acids from a broad range of sample types, allows researchers, in a 90-minute manual protocol, to obtain DNA that can provide strong performance in long-read sequencing applications. Data on this performance was demonstrated in a poster presented at this week's 2020 Advances in Genome Biology and Technology (AGBT) meeting, Promega said. When run through a long-read sequencing protocol, the DNA from the Promega kit produced very long maximum reads and high N50 values, indicating a higher proportion of large fragments in the sample.
Compared to available alternatives, Promega claimed that the Wizard kit purifies a "significantly higher" proportion of fragments over 125kb. The kit supports purification from a variety sample types including whole blood, bacterial cultures, cultured mammalian cells, and plant leaf tissue.