PCR Biosystems IsoFast Bst Polymerase Reagents

London-based PCR Biosystems has launched a range of IsoFast Bst polymerase reagents for rapid, robust, and sensitive DNA and RNA amplification. Through its nucleic acid strand displacement capabilities, Bst polymerase eliminates the need for the high-temperature denaturation step associated with traditional Taq enzymes, the company said. This enables faster time to result and facilitates DNA and RNA amplification in the field without specialized thermocycling equipment. The reagents can produce results over a wide dynamic range and sensitivities down to three copies of target DNA (10 fg). The new range of reagents can be used for a variety of techniques including whole-genome amplification, multiple displacement amplification, and isothermal amplification for applications such as environmental and food pathogen testing, clinical diagnostics, and next-generation sequencing.