Pacific Biosciences Microbial Multiplexing Kit
Pacific Biosciences has a launched a microbial multiplexing kit for its single-molecule sequencing instrument Sequel. The kit includes two new validated barcoded adapter kits and makes use of PacBio's new Sequel Binding/Sequencing Kit 2.1 and its analysis software SMRT Link version 5.1.0, according to the company. Multiplexing is done by incorporating a unique barcode for each microbe into the SMRTbell adapters after shearing genomic DNA. The process does not require PCR amplification, so epigenetic information is preserved.