Oxford Nanopore Technologies R10.3 Nanopore Chemistry

Oxford Nanopore Technologies has released flow cells using the R10.3 nanopore chemistry. It is currently available for MinIon and PromethIon sequencers and will be available for Flongle flow cells at a later time. The R10.3 chemistry, which is suitable for use with the LSK109 kit at launch, improves on the R10. Like the R10, it uses a pore with a longer barrel and a dual reader head, which improves resolution of homopolymers and consensus accuracy. Compared to the R10, it has increased throughput and capture, is compatible with PromethIon flow cells, has input amounts closer to the R9.4.1 chemistry, and has improved raw accuracy to match the R9.4.1 and to support enhanced variant calling.