Oxford Nanopore Technologies Direct cDNA (SQK-DC109), PCR-cDNA (SQK-PCS109) Kits
Oxford Nanopore Technologies has launched two new cDNA sequencing kits for its nanopore devices. The kits, called Direct cDNA (SQK-DC109) and PCR-cDNA (SQK-PCS109), generate sequence data for full-length cDNA and require less than five hours of sample preparation. The Direct cDNA kit does not require PCR and needs 100 ng of poly-A+ RNA. The PCR-cDNA kit has an input requirement of just 1 ng of polyA+ RNA and can start from total RNA. Users can obtain 10 to 15 million reads on a MinIon flow cell and more than 60 million reads on a PromethIon flow cell. According to the firm, the kits are available for the MinIon, GridIon, and PromethIon sequencers.