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Oxford Nanopore Technologies Direct cDNA (SQK-DC109), PCR-cDNA (SQK-PCS109) Kits

Oxford Nanopore Technologies has launched two new cDNA sequencing kits for its nanopore devices. The kits, called Direct cDNA (SQK-DC109) and PCR-cDNA (SQK-PCS109), generate sequence data for full-length cDNA and require less than five hours of sample preparation. The Direct cDNA kit does not require PCR and needs 100 ng of poly-A+ RNA. The PCR-cDNA kit has an input requirement of just 1 ng of polyA+ RNA and can start from total RNA. Users can obtain 10 to 15 million reads on a MinIon flow cell and more than 60 million reads on a PromethIon flow cell. According to the firm, the kits are available for the MinIon, GridIon, and PromethIon sequencers.

The Scan

Looking for Omicron

NPR reports that SARS-CoV-2 testing in the US has gotten better but also that some experts say more needs to be done to better track the Omicron variant.

Holmes Alleges Abuse

The Associated Press reports that Theranos' Elizabeth Holmes has testified at her wire fraud trial that her business and romantic partner abused her.

Bit More Diverse, But More to Do

While Black and Hispanic patients are more likely to participate in cancer clinical trials than previously, they are still underrepresented, according to US News & World Report.

PNAS Papers on Yeast Gene Silencing, Zika Virus Inhibition, Immunoglobulin Hypermutation

In PNAS this week: gene silencing in Saccharomyces cerevisiae, possible neuroprotective role for SHFL in a mouse model of Zika virus infection, and more.