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Oxford Nanopore PCR-cDNA and Direct cDNA Sequencing Kits

Oxford Nanopore Technologies has launched a PCR-cDNA Sequencing kit and a Direct cDNA Sequencing kit to prepare cDNA for nanopore sequencing. The PCR-cDNA kit requires a minimum input of 50 nanograms of RNA and the Direct cDNA kit requires at least 250 nanograms of RNA. Both kits can be used on any RNA with a polyA tail, and both are compatible with barcoding kits to multiplex samples.

The Scan

Team Tracks Down Potential Blood Plasma Markers Linked to Heart Failure in Atrial Fibrillation Patients

Researchers in BMC Genomics found 10 differentially expressed proteins or metabolites that marked atrial fibrillation with heart failure cases.

Study Points to Synonymous Mutation Effects on E. Coli Enzyme Activity

Researchers in Nature Chemistry saw signs of enzyme activity shifts in the presence of synonymous mutations in a multiscale modeling analysis of three Escherichia coli genes.

Team Outlines Paternal Sample-Free Single-Gene Approach for Non-Invasive Prenatal Screening

With data for nearly 9,200 pregnant individuals, researchers in Genetics in Medicine demonstrate the feasibility of their carrier screening and reflex single-gene non-invasive prenatal screening approach.

Germline-Targeting HIV Vaccine Shows Promise in Phase I Trial

A National Institutes of Health-led team reports in Science that a broadly neutralizing antibody HIV vaccine induced bnAb precursors in 97 percent of those given the vaccine.