Skip to main content

New England Biolabs NEBNext Enzymatic Methyl-seq

New England Biolabs has launched NEBNext Enzymatic Methyl-seq (EM-seq), an enzyme-based method for methylation sequencing that is an alternative to bisulfite sequencing. The EM-seq kit involves a two-step enzymatic conversion that first protects 5-methyl cytosine and 5-hydroxymethyl cytosine from deamination using TET2 and an oxidation enhancer, and then deaminates unmethylated cytosines, converting them to uracil. Because the process does not rely on chemical treatment, as with bisulfite conversion, the DNA is not damaged, according to the company. The kit includes the required reagents for EM-seq conversion and library preparation for sequencing on Illumina instruments.

The Scan

And For Adolescents

The US Food and Drug Administration has authorized the Pfizer-BioNTech SARS-CoV-2 vaccine for children between the ages of 12 and 15 years old.

Also of Concern to WHO

The Wall Street Journal reports that the World Health Organization has classified the SARS-CoV-2 variant B.1.617 as a "variant of concern."

Test for Them All

The New York Times reports on the development of combined tests for SARS-CoV-2 and other viruses like influenza.

PNAS Papers on Oral Microbiome Evolution, Snake Toxins, Transcription Factor Binding

In PNAS this week: evolution of oral microbiomes among hominids, comparative genomic analysis of snake toxins, and more.