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New England Biolabs Luna SARS-CoV-2 RT-qPCR Multiplex RUO Assay Kit

New England Biolabs has released the research-use-only Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, which enables multiplex testing of up to 94 samples in a single 96-well plate with the additional capacity to support sample pooling and high-throughput applications using 384-well plates. The kit detects two separate regions of the SARS-CoV-2 N gene (N1 and N2) in addition to the human RNase P gene internal control, based on sequences provided by the US Centers for Disease Control and Prevention. The probes have been modified to detect N1 and N2 via two different fluorophores — HEX and FAM, respectively — for simultaneous fluorescence detection on two different channels of a real-time instrument. To reduce the risk of carryover contamination between reactions, the master mix includes dUTP and a unique, thermolabile UDG that offers significant benefits over the standard E. coli UDG, NEB said.

The Scan

Another Resignation

According to the Wall Street Journal, a third advisory panel member has resigned following the US Food and Drug Administration's approval of an Alzheimer's disease drug.

Novavax Finds Its Vaccine Effective

Reuters reports Novavax's SARS-CoV-2 vaccine is more than 90 percent effective in preventing COVID-19.

Can't Be Used

The US Food and Drug Administration says millions of vaccine doses made at an embattled manufacturing facility cannot be used, the New York Times reports.

PLOS Papers on Frozen Shoulder GWAS, Epstein-Barr Effects on Immune Cell Epigenetics, More

In PLOS this week: genome-wide association study of frozen shoulder, epigenetic patterns of Epstein-Barr-infected B lymphocyte cells, and more.