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MilliporeSigma CRISPRi Whole-Genome Libraries and Pools

MilliporeSigma has launched its CRISPR inhibition (CRISPRi) whole-genome libraries and pools. CRISPRi is a useful tool for screening an entire genome, smaller gene family or individual targets. It modulates gene expression, allowing for robust loss-of-function studies, even in essential genes, with fewer off-target effects. The CRISPRi libraries libraries and pools were created by researchers at the University of California, San Francisco, and were further optimized by MilliporeSigma, resulting in improved algorithm design and scaffolding that has been proven to increase gene knock down, even in difficult-to-repress targets, the company said.

The libraries are fully customizable, contain the top five guide RNAs (gRNAs) and a subpool of five additional gRNAs per gene for a total of 10 gRNAs targeting every gene. They have defined subpanels to target specific gene groups including the druggable genome, cancer and apoptosis, membrane proteins, stress and proteostasis genes, and others. And the system includes 10x Genomics-compatibility for downstream single cell analysis, the firm said.

MilliporeSigma's gene modulation offering also includes CRISPR activation (CRISPRa) whole-genome libraries and pools.