Skip to main content
Premium Trial:

Request an Annual Quote

Lexogen RiboCop rRNA Depletion Kits for Bacteria

Lexogen has developed a set of new RiboCop rRNA Depletion Kits for bacteria, which it said efficiently removes 23S, 16S, and 5S rRNA from mixed bacterial samples and monocultures. According to the firm, RiboCop is offered as a standalone kit with the option to choose from three optimized probe mixes for depletion of either rRNA from mixed bacterial samples, or Gram-negative or Gram-positive bacteria grown in monoculture.

The technology uses a set of affinity probes designed for specific depletion of rRNA sequences, and, according to Lexogen, the probe design minimizes off-target effects that can distort NGS data quality. The kits work with total RNA input amounts as low as 1 ng and up to 1 μg, depending on sample composition and the chosen depletion probe mix. RiboCop does not include any enzymatic reactions or mechanical shearing steps and can process intact as well as degraded material, including FFPE, resulting in intact, full-length transcripts. Resulting rRNA-free samples are then suitable for a variety of downstream RNA analysis methods including RNA-seq library preparation approaches.

The protocol is also automation-friendly, the firm added.

The Scan

Tara Pacific Expedition Project Team Finds High Diversity Within Coral Reef Microbiome

In papers appearing in Nature Communications and elsewhere, the team reports on findings from the two-year excursion examining coral reefs.

Study Examines Relationship Between Cellular Metabolism, DNA Damage Repair

A new study in Molecular Systems Biology finds that an antioxidant enzyme shifts from mitochondria to the nucleus as part of the DNA damage response.

Stem Cell Systems Target Metastatic Melanoma in Mouse Model

Researchers in Science Translational Medicine describe a pair of stem cell systems aimed at boosting immune responses against metastatic melanoma in the brain.

Open Pediatric Brain Tumor Atlas Team Introduces Genomic Data Collection, Analytical Tools

A study in Cell Genomics outlines open-source methods being used to analyze and translate whole-genome, exome, and RNA sequence data from the Pediatric Brain Tumor Atlas.