Lexogen has developed a set of new RiboCop rRNA Depletion Kits for bacteria, which it said efficiently removes 23S, 16S, and 5S rRNA from mixed bacterial samples and monocultures. According to the firm, RiboCop is offered as a standalone kit with the option to choose from three optimized probe mixes for depletion of either rRNA from mixed bacterial samples, or Gram-negative or Gram-positive bacteria grown in monoculture.
The technology uses a set of affinity probes designed for specific depletion of rRNA sequences, and, according to Lexogen, the probe design minimizes off-target effects that can distort NGS data quality. The kits work with total RNA input amounts as low as 1 ng and up to 1 μg, depending on sample composition and the chosen depletion probe mix. RiboCop does not include any enzymatic reactions or mechanical shearing steps and can process intact as well as degraded material, including FFPE, resulting in intact, full-length transcripts. Resulting rRNA-free samples are then suitable for a variety of downstream RNA analysis methods including RNA-seq library preparation approaches.
The protocol is also automation-friendly, the firm added.