Cellecta has commercially launched its dual-sgRNA libraries designed for CRISPR activation (CRISPRa) and CRISPR interference/repression (CRISPRi) genetic screens. With the modified systems, the firm said it has re-engineered the standard knockout capacity to modulate gene activitiy. The variations extend the types of genetic screenings possible.
According to Cellecta, its dual-sgRNA CRISPRa and CRISPRi libraries improve single-sgRNA library activity because each construct expresses two different sgRNA binding distinct sites on the promoter of each gene target. The firm believes this advantage increases the likelihood that each targeted gene will be activated or repressed above a given threshold when compared to libraries that only have a single sgRNA targeting a gene promoter.