Logo

Fluidigm REAP-Seq Protocol for C1 System

Feb 04, 2019

Fluidigm has introduced a REAP-seq (RNA expression and protein sequencing) protocol for use with its C1 sample prep system for single-cell genomics. C1 REAP-seq enables characterization of unique cellular subtypes and functional states by measuring the expression of both cellular proteins and RNAs. Users can pair the protocol with functional imaging assays that measure differences in cell size, morphology, or phenotype within the clear C1 microfluidic cell chambers. The firm developed C1 REAP-seq in collaboration with Merck for co-detection of both cellular protein and RNA using microfluidic technology.

Breaking News
The Scan

Positive Framing of Genetic Studies Can Spark Mistrust Among Underrepresented Groups

Researchers in Human Genetics and Genomics Advances report that how researchers describe genomic studies may alienate potential participants.

Small Study of Gene Editing to Treat Sickle Cell Disease

In a Novartis-sponsored study in the New England Journal of Medicine, researchers found that a CRISPR-Cas9-based treatment targeting promoters of genes encoding fetal hemoglobin could reduce disease symptoms.

Gut Microbiome Changes Appear in Infants Before They Develop Eczema, Study Finds

Researchers report in mSystems that infants experienced an enrichment in Clostridium sensu stricto 1 and Finegoldia and a depletion of Bacteroides before developing eczema.

Acute Myeloid Leukemia Treatment Specificity Enhanced With Stem Cell Editing

A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy.