Cartana In Situ Sequencing Reagent Kits
Cartana has launched a new range of in situ sequencing reagent kits designed to increase the efficiency and enable ISS on virtually any tissue sample. The new kits contain improved probing and sequencing technologies and come with easy-to-follow protocols for library preparation and sequencing. The HS Library Preparation Kit is used to probe directly up to 600 RNA targets in either fresh/fixed frozen or FFPE samples without the need for cDNA synthesis, and generates barcoded in situ sequencing libraries with higher efficiency compared to a previous cDNA-based library preparation approach, initially published in 2013, Cartana said. Furthermore, the new ISS kit sequences the barcodes in the libraries directly in situ, with enhanced signal-to-noise ratio and specificity compared to the previous state-of-the-art ISS technology. The new kit contains six sequencing cycles and decodes up to 600 barcodes using a standard fluorescent microscope.