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Agilent SureGuide Pooled CRISPR Libraries

Agilent Technologies has launched the first expansion of its SureGuide pooled CRISPR libraries for functional genomics, and is now offering pooled libraries for CRISPR activation and interference. The SureGuide CRISPR libraries are synthesized using Agilent's SurePrint DNA synthesis platform, which simultaneously prints billions of DNA bases. The new CRISPR a/i libraries incorporate gene targets developed by researchers at UCSF, Agilent said.

SureGuide CRISPR a/i libraries are offered in both Ready-to-Clone and Ready-to-Amplify formats. With Ready-to-Clone libraries, scientists can specify the sequence of each guide in the library or choose from validated predefined subsets of the genome, such as kinases or cancer genes. With Ready-to-Amplify libraries, investigators can fully design every aspect of the library, including the tracer sequence, allowing for the use of alternative delivery systems, guide structures, and cloning approaches, while targeting any organism of choice.