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Biotium EvaRuby Dye for qPCR and HRM

Biotium has introduced its EvaRuby Dye for qPCR and HRM (high-resolution melting). This spectrally unique red fluorescent intercalating dye can be used to expand multiplexing options for probe-based qPCR. It is compatible with approximately 470 nm excitation and 610 nm emission filters. Its excitation is by the same green/blue channel as EvaGreen Dye, and detection is in the orange (JUN or ROX) channel. As a result, it has minimal cross-talk in standard probe detection channels and enables the combination of dye-based and probe-based qPCR in a single reaction, the company said. In addition, the dye is validated for HRM analysis and may be used as an internal control for probe-based qPCR to detect non-specific amplification that goes undetected by target-specific probes, Biotium added.

Meridian Bioscience Air-Dryable LAMP Mixes

Meridian Bioscience has launched its Air-Dryable LAMP Mix and Air-Dryable RT-LAMP 1-Step Mix. The molecular master mixes are designed for fast isothermal amplification of nucleic acid sequences to facilitate the development of point-of-care ambient temperature-stable tests, and to accelerate diagnosis. The mixes cut the usual sample-to-result time of loop-mediated isothermal amplification tests in half, the company said. Both mixes allow more sample to be added and have pre-optimized buffer systems, including enzymes, stabilizers, enhancers, and nucleotides.

Pacific Biosciences HiFiViral SARS-CoV-2 Kit and Upgraded Microbial WGS Application

Pacific Biosciences has launched the HiFiViral SARS-CoV-2 kit, an end-to-end solution for COVID-19 surveillance, and an upgraded HiFi microbial whole-genome sequencing application. The SARS-CoV-2 kit uses molecular inversion probes, rather than PCR-based amplification and provides companion SMRT Link v10.2 software. It can process up to 384 samples at a time. The microbial WGS application adds HiFi sequencing and doubles the recommended multiplexing capacity to 96.

Blackhawk Genomics HerediSeq, PanSeq

Blackhawk Genomics has launched two genomic analysis kits, HerediSeq and PanSeq, designed to detect structural variants and elucidate difficult-to-characterize genomic regions using long-read sequencing. Both assays use CRISPR/Cas9 technology to enrich specific genomic regions prior to sequencing, without the need for amplification.  

HerediSeq chemistry resolves deletions and duplications, repetitive regions and pseudogenes, allowing for easy and accurate identification of germline structural variants in genes associated with the increased risk of hereditary cancer and cardiac diseases as well as those relevant in carrier screening applications. PanSeq uses targeted enrichment for rapid detection of microbial, fungal, and viral species, plus treatment resistance markers.

Currently, three HerediSeq kits are available targeting cancer, cardiac diseases, and carrier screening content. Up to 24 samples can be sequenced in a single flow cell or run, and sample preparation takes less than a day. Each kit can process 96 samples. PanSeq kits can process 96 samples, all in a single run, with data available in less than a day from sample receipt. 

Bio-Rad Laboratories StarBright UltraViolet 400 Dye for Flow Cytometry

Bio-Rad Laboratories has launched the StarBright UltraViolet 400 Dye for flow cytometry, the first in a range of fluorescent nanoparticles designed for use with a UV laser in flow cytometry applications. The dye offers exceptional brightness with narrow excitation and emission profiles, making it suitable for use in multicolor flow cytometry panels, Bio-Rad said. The entire range of StarBright Dyes are conjugated to highly validated flow antibodies and are compatible with most flow cytometers and experimental protocols, the company added.

Thermo Fisher Scientific Applied Biosystems QuantStudio 7 Pro Dx Real-Time PCR System

Thermo Fisher Scientific has launched the Applied Biosystems QuantStudio 7 Pro Dx Real-Time PCR System, a standalone qPCR platform that the company said is versatile, highly expandable, and easy to use. Designed to support in vitro diagnostic applications and the development of molecular diagnostic assays, the QuantStudio 7 Pro Dx system has sensitivity down to 1.5-fold in singleplex reactions and 10 logarithmic units of linear dynamic range. The platform supports interchangeable 96-well and 384-well blocks and automation compatibility, and for molecular laboratories developing and running their own tests, there are embedded regulatory controls to support traceability and reduce the risk of unauthorized use or potential misuse.

Other key system features include: security, auditing, and e-signature functionalities to assist in compliance with regulatory requirements and support multiple clients; software designed to guide users through workflow steps for test development or IVDs; the ability to create and manage assay definition files (ADFs) for a specific assay, panel, or test that incorporate all of the qPCR setup and analysis parameters required for a particular assay; a small footprint that doesn't require an attached computer, with a graphical user interface that enables end-to end workflows from the touchscreen; a six-color, decoupled OptiFlex filter system with bright white LED; hands-free functionality to enable the performance basic operations using voice commands; and smart support and smart help to report issues or request instrument services to reduce downtime.The system also uses up to 54 percent less material, generates 91 percent less waste during calibration, and uses up to 26 percent less energy than comparable instruments, the company said.

The US Food and Drug Administration has listed the QuantStudio 7 Pro Dx Real-Time PCR System as a class II medical device, Thermo Fisher added.

Bio-Rad Laboratories SARS-CoV-2 Controls

Bio-Rad Laboratories has launched the SARS-CoV-2 S gene alpha, beta, gamma, and epsilon variant controls as part of its Exact Diagnostics line of molecular quality control products for research testing. The controls are intended to be validated as independent external run controls and used for research testing with molecular assays targeting mutations in the SARS-CoV-2 Spike (S) gene. They are intended to be used with assays detecting SARS-CoV-2 variant RNA and to be processed in the same manner as patient specimens to monitor all the steps of molecular assays, Bio-Rad said. The variant S genes and human gDNA are internally targeted to 200,000 copies/ml and 75,000 copies/ml, respectively, using Bio-Rad Droplet Digital PCR to ensure lot-to-lot consistency, the company added.

GenScript GenWand Double-Stranded DNA Service

GenScript has launched the GenWand Double-Stranded DNA Service for the manufacture of CRISPR knock-in homology-directed repair templates in T cell engineering. Closed-end dsDNA enables researchers to perform gene knock-in with higher efficiency and lower toxicity than traditional PCR methods and is ideally suited for large-scale screening and scale-up in cell and gene therapy, the company said. The service manufactures covalently closed-end linear DNA to help increase the stability of dsDNA by limiting endonucleases' digestion of the dsDNA once entering cells. It also mitigates non-homologous end joining risk and increases knock-in accuracy when using CRISPR homology-directed repair machinery, GenScript said. Made via a proprietary production process, GenScript's dsDNA is available from micrograms to grams, and in 2-10 kb sequences. Various quality grades are available from research grade to basic GMP, the company said.

Fluidigm Biomark X

Fluidigm has launched BioMark X, the latest generation of its Biomark microfluidic real-time PCR platform. Biomark X integrates the company's Juno microfluidic sample preparation platform with the company's current BioMark HD instrument into a single sample-to-answer platform, the company said. The integrated system can be used for a variety of applications including genotyping, sample ID, strain ID, conservation biology, personal genomics, gene expression, pathogen detection, pharmacogenomics assays, and transplant prognostics, according to Fluidigm. The platform also supports development and creation of custom assays, and the ability to scale the number of targets based on need. According to the company's website, BioMark X supports the company's 48.48 Dynamic Array Integrated Fluidic Circuit (IFC), enabling 2,304 reactions using 48 samples and assays; and the 96.96 Dynamic Array IFC, enabling 9,216 reactions using 96 samples and assays.

Fluidigm said the platform is in a phased launch with deliveries expected to commence in the first quarter of 2022.

Genes2Me RT-Direct Multiplex RT-PCR Kit for COVID-19

Genes2Me, an India-based IVD manufacturer and genetic diagnostics company, announced the global launch of the RT-Direct Multiplex RT-PCR kit for COVID-19. The kit, which received CE marking in August, covers three target genes specific to SARS-CoV-2 and has been validated with 100 percent sensitivity and specificity, the company said. Results can be obtained in about 40 minutes from sample to report without the need for prior RNA extraction, Genes2Me said. The kit is compatible with any commercial real-time PCR instrument.

The Scan

Comfort of Home

The Guardian reports that AstraZeneca is to run more clinical trials from people's homes with the aim of increasing participant diversity.

Keep Under Control

Genetic technologies are among the tools suggested to manage invasive species and feral animals in Australia, Newsweek says.

Just Make It

The New York Times writes that there is increased interest in applying gene synthesis to even more applications.

Nucleic Acids Research Papers on OncoDB, mBodyMap, Genomicus

In Nucleic Acids Research this week: database to analyze large cancer datasets, human body microbe database, and more.