New England Biolabs has launched two new RNA depletion products to support next-generation RNA sequencing.
The NEBNext Globin & rRNA Depletion Kit (human/mouse/rat) depletes adult, fetal, and embryonic globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), as well as cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S).
The NEBNext rRNA Depletion Kit (bacteria) removes the abundant bacterial rRNAs (5S, 16S, and 23S) resulting in the enrichment of bacterial mRNAs and non-coding RNAs of interest. This method is targeted to both gram-positive and gram-negative organisms, and is effective with RNA from monocultures or mixed bacterial species, such as those used in metatranscriptome studies, NEB said.
The new kits complement the NEBNext products for RNA library preparation, including the Ultra II Directional RNA Library Prep Kit for Illumina and the NEBNext Library Quant Kit for Illumina, as well as the Monarch Total RNA Miniprep Kit, the company said.