Senior Research Assistant (Induced Pluripotent Stem Cells)

Organization
Joslin Diabetes Center
Job Location
One Joslin Place
Boston, MA 02215
Salary
Based on experience
Job Description

The Senior Research Assistant, under general supervision of the GEC core facility directors, carries out independently the daily activities of the core facility.

 Responsibilities:

  • Derives fibroblasts from skin biopsies.
  • Derives, maintains, validates, distributes and maintains accurate records of use of induced pluripotent stem cell lines.
  • Performs microbiological screening of iPSC lines
  • Performs gene-editing services, including CRISPR/Cas9 mediated genome modification of iPSCs and other cell lines.
  • Characterizes new lines by standard molecular biological techniques (including DNA and RNA extraction, PCR, RT-PCR), by immunophenotypic analysis (including cytostaining and flow cytometry) and by functional assays (in vitro and in vivo differentiation).
  • Performs quality control of cell lines.
  • Instructs others in basic laboratory and tissue culture techniques and iPS- and gene editing-specific technologies, including the organization and teaching of training workshops for researchers interested in learning techniques relevant to iPSC derivation, culture and gene-editing.
  • Performs related laboratory maintenance and management, including maintaining and cleaning equipment, interfacing with vendors, ordering and keeping an adequate inventory of laboratory supplies, tracking core usage and invoicing participating laboratories.
  • Assists in the preparation of progress reports, grant applications, and manuscripts relevant to the core’s activities.

  

Requirements

Qualifications:

  • Bachelors or Masters degree in biology or related field.
  • Minimum 2 year of experience preferably with iPS cells and/or CRISPR gene editing technology
  • Previous experience with primary cell cultures and sterile laboratory technique is required.
  • Exceptional organizational and record keeping skills and excellent attention to detail are an essential component of this position.
  • Ability to work pro-actively and in a collaborative, team-oriented environment. Some weekend flexibility will be required.
  • Computer experience is necessary.

 Joslin Diabetes Center is an Equal Opportunity Employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability status, protected veteran status or any other status protected by law.

About Our Organization

Joslin Diabetes Center — dedicated to conquering diabetes in all of its forms — is the global leader in diabetes research, care and education. Joslin research is at the forefront of discovery aimed at preventing and curing diabetes. The Joslin clinic develops innovative patient therapies that immeasurably improve the lives of people with diabetes. Joslin is an independent, non-profit institution affiliated with Harvard Medical School, and one of only 11 NIH-designated Diabetes Research Centers in the U.S.

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