Research Technician II-III, Molecular Biology

Organization
Fred Hutchinson Cancer Research Center & Seattle Cancer Care Alliance
Job Location
Seattle, WA
Job Description

Cures Start Here.
At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch's pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation's first and largest cancer prevention research program, as well as the clinical coordinating center of the Women's Health Initiative and the international headquarters of the HIV Vaccine Trials Network.

Careers Start Here.
- Assist in the design, implementation and interpretation of human gene therapy experiments using chimeric antigen receptor (CAR)-expressing T lymphocytes to target B cell lymphomas.
- Perform detailed immunophenotyping of CAR T cells by flow cytometry for patient-derived CAR T cell products for human clinical trials, and evaluate post-treatment patient blood, marrow, and lymph node samples from CAR T cell-treated patients to evaluate persistence and phenotype of infused cells as well as evaluation of tumor cell phenotypes.
- Maintain human lymphoid cells in long-term cell culture; transduce them with retroviral or lentiviral vectors encoding chimeric human fusion genes; select the genetically modified cells by immunomagnetic sorting; and expand and clone them under both standard laboratory and cGMP conditions.
- Monitor the effectiveness of transduction and clone expansion by PCR, Western blotting, flow cytometry, and cytotoxicity assays using the genetically modified T cells.
- Conduct in vitro assays testing the function of CAR T cells, including proliferation, cytotoxicity, and cytokine secretion.
- Perform mouse studies to test the efficacy of CAR T cells in immunodeficient mouse xenograft studies.
- Monitor T cell persistence with samples obtained by retroorbital bleeding, and evaluate tumor responses in mouse models using bioluminescent imaging.
- Apply molecular cloning methods to generate new lentiviral and retroviral constructs.
- Produce research-grade lentivirus or retrovirus by transfecting packaging cells with lentiviral and retroviral vector plasmids, and concentrating and purifying lentiviral supernatant.
- Perform quality control for sterility, and assess the presence of endotoxin, viruses, bacteria, fungi, mycoplasma and other contaminants in transduced cell lines.
- Assist other lab personnel with immunocompetent mouse experiments, including injections of tumor cells, T cells, and chemotherapeutic agents, tumor measurements, and harvesting of mouse blood, marrow, and lymphoid organs.
- Assist in preparation of manuscripts for publication and presentation of research results at national meetings.
- Serve as a lab manager, including ensuring adequate lab supplies are in stock, coordinating lab chores among lab members, and maintaining organized freezer inventories.
- Perform other duties as assigned by supervisor.

Required:
Bachelor's degree in biological or chemical sciences.

Desirable:
2 years' experience in relevant research.

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