Research Technician I, HIV Vaccine Trials Network

Fred Hutchinson Cancer Research Center & Seattle Cancer Care Alliance
Job Location
Seattle, WA
Job Description

Cures Start Here
At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch's pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer with minimal side effects. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation's first and largest cancer prevention research program, as well as the clinical coordinating center of the Women's Health Initiative and the international headquarters of the HIV Vaccine Trials Network.

Careers Start Here.
This research opportunity is with the HIV Vaccine Trials Network (HVTN). The technician will perform studies to identify and characterize T-cell responses induced in candidate vaccine recipients participating in clinical trials of investigational HIV or related infectious disease vaccines. S/he will be responsible for processing blood or other body fluids from volunteers, performing the assays, and collecting and analyzing the data. S/he may work with the clinical staff from the FHCRC HIV vaccine Trials Unit (HVTU) and with sites within the HVTN to coordinate specimen transfer and data analysis. S/he will assist in the development and maintenance of laboratory quality control (QC) methods and records. S/he will interpret and report on experimental results and assist with data presentation for conferences and manuscripts.

- Prepare and maintain reagents, stocks, and cultures necessary to perform the work
- Order and maintain supplies
- Prepare PBMC or purified T-lymphocyte populations
- Perform ELISPOT and LUMINEX assays for cytokine producing cells
- Perform flow cytometric assays, including intracellular cytokine staining and MHC/peptide tetramer staining
- Perform in vitro cell manipulations, including peptide stimulations
- Collect and analyze data
- Maintain records for experiments and quality control for the laboratory
- Maintain a high level of accuracy for all work
- Work well as team-member
- Operate and carry out basic maintenance and QC of laboratory equipment
- Perform other duties as assigned

A bachelor's degree in a biological science and at least 6 months of bench research experience. Experience with immunology, cellular immunologic assays, tissue culture, multi-parameter flow cytometry, microscopy, PCR, DNA extractions, and micropipetting are preferred. Experience in a GCLP/GLP setting or clinical experience, such as CLIA, CAP, or JACHO, with strong documentation skills is also desirable. Motivation and adaptability and ability to be a strong team player are essential, as is a keen interest in the HIV vaccine field. Applicants must be willing to work within the core hours of 8am-5pm on weekdays and to work occasional evenings and weekends as needed. Individual must also be comfortable working with biohazardous/infectious agents (e.g., bloodborne pathogens, including HIV-1).

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