This position is available for a motivated full time Research Technician in the Van Andel Research Institute’s Laboratory of Chromatin and Epigenetic Regulation. Under the direction of Dr. Scott Rothbart, the lab studies basic molecular and cellular mechanisms controlling chromatin accessibility, interaction and function. Positioned in the VARI Center for Epigenetics, the candidate will be engrossed in an energetic, interactive and fast-paced environment at the forefront of research into epigenetic mechanisms and therapeutics in human health and disease.
The chosen candidate will assist senior personnel with experiments and lab operations, and will be expected to participate actively in experimental planning and execution, as well as data analysis and presentation. The candidate will also have the opportunity to conduct independent research projects and author on scientific publications.
The position will require an individual to be responsible for the following minimal tasks:
Ø Maintain and manipulate mammalian cell lines with good sterile technique
Ø Perform molecular biology experiments including cloning, PCR, mutagenesis and DNA analysis
Ø Express and purify recombinant proteins from bacteria
Ø Analyze proteins by SDS-PAGE and western blotting
Ø Prepare buffers, solutions, reagents, media and stains necessary for experimental procedures
Ø Inventory laboratory supplies and equipment and prepare requisitions for approval
Ø Perform other laboratory duties as requested
Additional skills that are desired, but not required:
Ø Experience with biophysical assays (e.g., fluorescence polarization and isothermal titration calorimetry)
Ø Proficiency in techniques to analyze DNA methylation and histone post-translational modifications
Ø Familiarity with sample preparation for mass-spec based proteomics
Ø Experience with fabrication and/or use of microarrays