Research Techn II

Fred Hutchinson Cancer Research Center & Seattle Cancer Care Alliance
Job Location
Seattle, WA
Job Description

Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch's pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation's first and largest cancer prevention research program, as well as the clinical coordinating center of the Women's Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.

A qualified and motivated candidate is sought to join the Optides Program (Project Violet) at the Fred Hutch. Our program is focused on the discovery and advancement of new treatments for pediatric brain cancers and other devastating diseases. Building on the success of Tumor Paint, we have developed a novel way of making knottin peptides, giving us unique access to this mid-sized medicine space. We are looking for a technician to join our growing team of protein engineers to help with technology development as well as protein production using mammalian and insect cell-based platforms. Join us and make a difference!

The responsibilities and duties expected of this position are to support all aspects of the production, purification and characterization of novel proteins using mammalian and insect expression platforms. This includes the ability to prepare expression vectors using standard molecular biology techniques including plasmid DNA preparation, restriction enzyme digestion, Gibson assembly and DNA sequencing. This will require meticulous organizational skills and attention to detail as the group is now adopting high-throughput techniques. Other responsibilities will include mammalian tissue culture, lentivirus generation and protein purification using affinity and size exclusion chromatography under endotoxin-free conditions. Applicants are expected to be able to design DNA primers, clone target constructs, run SDS-PAGE and polyacrylamide gels and to organize their data into reports that are used to guide our drug discovery process with minimal supervision. Candidates with previous molecular biology and protein production experience will be strongly considered.

The ideal candidate for this position is a research technician with a BS or MS in Biology, Chemistry or Biochemistry and at 1-2 years of technical lab experience in an academic or industry setting. The position requires a high level of organization and the ability to manage multiple projects at once. The candidate must be skilled in PCR, cloning, cell culture, protein expression and purification. A working familiarity with ÄKTA chromatography systems and experience with mammalian or insect expression systems is highly desired. The candidate must have a proven ability to manage independent research and good written and oral communication skills.

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