The University of Wisconsin Biotechnology Center is a fee-for-service core facility. The applicants would be expected to support the services of the TGF and TAF. A successful employee demonstrates efficient time management skills in addition to strong attention to detail in carrying out lab and animal related tasks.
The duties will include, but not necessarily be limited to:
30%: Synthesis and purification of CRISPR/Cas9 genome editing reagents by in vitro transcription, nucleotide quantification, traditional cloning, and Gibson assembly
30%: Screening of genome editing projects by PCR, Sanger sequencing, restriction digests and high-throughput 96-well PCR
25%: Animal model support including pre-implantation embryo collection, tissue sampling, breeding and general animal husbandry of mice and rats
10%: Research and Development
5%: General laboratory maintenance tasks such as ordering supplies, communication between users of TGF and TAF, oversee undergraduate student assistants
The prefix and salary will be determined based on years of experience and academic preparation. Some weekend, holiday and after hours work will be required.