Research Scientist I

Broad Institute
Job Location
Cambridge, MA
Job Description

The Broad Institute of MIT and Harvard is the world’s leading biomedical research institute dedicated to the bold mission of using the full power of genomics to transform the understanding and treatment of disease. The Broad Institute seeks to describe all of the molecular components of life and their connections; discover the molecular basis of major human diseases; develop effective new approaches to diagnostics and therapeutics; and disseminate discoveries, tools, methods, and data openly to the entire scientific community.

Founded in 2004 by Eric Lander and philanthropists Eli and Edythe L. Broad, the Broad Institute includes faculty, professional staff, and students from throughout the MIT and Harvard biomedical research communities and beyond, with collaborations spanning more than a hundred private and public institutions in more than 40 countries worldwide.

The candidate will work independently under the guidance of Drs. Mark Daly and Daniel MacArthur of the Analytic and Translational Genetics Unit, which is jointly based at the Broad Institute of Harvard and MIT and Massachusetts General Hospital. He/she will be based primarily at the Broad Institute, and will conduct and coordinate multi-disciplinary scientific research exploring the functional impact of human genetic variation identified in large-scale sequencing studies of both rare and common disease.

The Research Scientist will lead the development and implementation of in vitro assays for the functional consequences of genetic variation identified in disease patients, using models of primary and stem-cell derived human cells as well as genetically engineered mice. He/she will develop robust cellular models of relevant tissues, including skeletal muscle, implement technologies to engineer these models to contain variants of interest (using both construct transfection and CRISPR/Cas9 engineering), and establish functional assays to assess the impact of these variants on pertinent biological pathways such as membrane repair or immune responsiveness.

The Research Scientist will also have the responsibility of managing collaborations within and between the Stanley Center and outside academic and industry/pharmaceutical partners. He/she will be responsible for independently initiating and developing new projects, concepts and methodologies.

Specific responsibilities include:
- Communicating effectively with a multidisciplinary team including informaticians, statistical geneticists and computational biologists to identify targets of potential interest for functional analysis from very large data-sets of human exome and genome sequencing data.
- Leading the development of robust in vitro cellular models of skeletal muscle and immune cell types, potentially including establishment and differentiation of human iPS cell lines.
- Maintaining a number of established cell lines for ongoing functional analysis.
- Using both transfection and CRISPR/Cas9 technology to engineer cells to express specific mutant versions of genes of interest.
- Characterizing the function of candidate risk genes and variants using technology within the lab, as well as in close and ongoing collaborations with other Broad laboratories and platforms.
- Investigating and implementing new technologies based on scientific needs.
- Managing internal and external collaborations relevant to the mission of the Analytic and Translational Genetics Unit.
- Establishing formal protocols for these experiments to allow them to be scaled to a high-throughput setting and adopted by other members of the research team.
- Managing and mentoring a growing team of junior scientists, under the guidance of Drs. Daly and MacArthur, including supervision of 1-3 direct reports.
- Writing scientific papers, reports, and presentations, and contributing to grant proposals.
- Participating in external activities, including the presentation of work at both national and international conferences.
- Required: PhD in molecular genetics, molecular biology or cell biology.
- Preferred: PhD with experience in the biology of either skeletal muscle or immune/inflammatory diseases
- 0+ years of postdoctoral research experience in a research or academic environment following receipt of graduate degree.
- Demonstrated technical proficiency in the analysis of either cellular or animal models of human diseases.
- Demonstrated technical proficiency in basic molecular biology techniques, including PCR, immunohistochemistry and Western blotting.
- Demonstrated success in working on complex novel problems, often integrating data from multiple disciplines.
- Demonstrated success in technical proficiency, scientific creativity, collaboration with others and independent.
- Publication and presentation record commensurate with experience.
- Proven record of significant contributions to research projects as individual contributor and as a collaborator.

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