Research Associate/Sr. Research Associate - Genomic Technologies Group - 80675
The Department of Energy(DOE) Joint Genome Institute (JGI), a division of the Lawrence BerkeleyNational Laboratory (Berkeley Lab) has an exciting opportunity available for an experienced Research Associate to join the Genomic Technologies group and work on the development and implementation of technologies for bacterial gene function annotation. Specifically we aim to use transposon mutagenesis coupled with high through put sequencing (Tn-Seq) to study the functional consequences of gene mutants on a genome-wide (For an overview and example, please see PMID: 23712350 and PMID:22125499). We have recently developed an improved Tn-Seq protocol, enabling its application on extremely high throughput scales. We now plan to implement this technology to: i) explore bacterial functional diversity, and ii) identify novel gene functions with relevance to specific biological questions, including those in the areas of bioenergy, carbon cycling, and bioremediation.
The selected candidate will be hired as at Research Associate/Sr. Research Associate classification depending on the candidate's level of skills and experience.
Under supervision, the RA /SRA will apply basic molecular biology and microbiology techniques to assist in all aspects of this project. These include: culturing of bacteria; development of transposons and transposon delivery systems; transposon mutagenesis; high throughput growth assays of mutant populations; isolation of nucleic acids; preparation of DNA for sequencing (Tn-Seq and Bar-Seq protocols) and development of new experimental approaches for studying genome function. This is a highly collaborative position requiring effective communication with experimental and computational scientists across the contributing research groups.
Specific Job Duties
• Culturing and mutagenesis of bacteria and archaea
• Cloning and development of molecular reagents for transposon mutagenesis
• Prepare genomic DNA for next-generation sequencing assays.
• Develop genomics applications utilizing new sequencing technologies
• Routine DNA sequence analysis.
• Keep detailed laboratory notebook and upload data in relevant databases.
• Prepare reports and standard operation procedures as requested.
• Participation and reporting in research group meetings.
Essential duties for Sr. Research Associate -
In addition to the job duties described above, the Sr. Research Associate will:
• Operate at a higher level of independence and responsibility when carrying out assignments.
• Serve as a technical resource to other members in the group and recommend process and database improvements.
• Assist in project coordination, mentoring RA’s and students and troubleshooting more advanced pathway optimization projects and processes.