Research Associate/Sr. Research Associate - Genomic Technologies Group

Lawrence Berkeley National Laboratory
Job Location
Walnut Creek, CA 94598
Job Description

Research Associate/Sr. Research Associate - Genomic Technologies Group - 80675
Organization:  GN-Genomics

The Department of Energy(DOE) Joint Genome Institute (JGI), a division of the Lawrence BerkeleyNational Laboratory (Berkeley Lab) has an exciting opportunity available for an experienced Research Associate to join the Genomic Technologies group and work on the development and implementation of technologies for bacterial gene function annotation. Specifically we aim to use transposon mutagenesis coupled with high through put sequencing (Tn-Seq) to study the functional consequences of gene mutants on a genome-wide (For an overview and example, please see PMID: 23712350 and PMID:22125499). We have recently developed an improved Tn-Seq protocol, enabling its application on extremely high throughput scales. We now plan to implement this technology to: i) explore bacterial functional diversity, and ii) identify novel gene functions with relevance to specific biological questions, including those in the areas of bioenergy, carbon cycling, and bioremediation.
The selected candidate will be hired as at Research Associate/Sr. Research Associate classification depending on the candidate's level of skills and experience.
Under supervision, the RA /SRA will apply basic molecular biology and microbiology techniques to assist in all aspects of this project. These include: culturing of bacteria; development of transposons and transposon delivery systems; transposon mutagenesis; high throughput growth assays of mutant populations; isolation of nucleic acids; preparation of DNA for sequencing (Tn-Seq and Bar-Seq protocols) and development of new experimental approaches for studying genome function. This is a highly collaborative position requiring effective communication with experimental and computational scientists across the contributing research groups.
Specific Job Duties
• Culturing and mutagenesis of bacteria and archaea
• Cloning and development of molecular reagents for transposon mutagenesis
• Prepare genomic DNA for next-generation sequencing assays.
• Develop genomics applications utilizing new sequencing technologies  
• Routine DNA sequence analysis.

• Keep detailed laboratory notebook and upload data in relevant databases.
• Prepare reports and standard operation procedures as requested.
• Participation and reporting in research group meetings.

Essential duties for Sr. Research Associate -
In addition to the job duties described above, the Sr. Research Associate will:
• Operate at a higher level of independence and responsibility when carrying out assignments.
• Serve as a technical resource to other members in the group and recommend process and database improvements.
• Assist in project coordination, mentoring RA’s and students and troubleshooting more advanced pathway optimization projects and processes.


• Excellent knowledge of basic microbiology, molecular biology and genetics concepts.
• Experience with basic microbiology and molecular biology techniques, especially bacterial growth and cloning.
• Experience with DNA sequencing technologies.
• Excellent analytical, organizational and multi-tasking skills.
• Proficiency in maintaining laboratory notes, reports and operating procedures.
• Good problem solving skills and a clear understanding of methods development processes.
• Able and willing to learn and implement new experimentation techniques.
• Due to highly collaborative nature of position, good interpersonal and communications skills are essential.

Essential qualifications for the Sr. Research Associate -
In addition to the qualifications described above, the Sr. Research Associate will have:
• Bachelor’s preferred and two to five years of demonstrated lab experience in Biology, Microbiology, Genetics or related field with increasing responsibility, or an MS/Ph.D Degree.
• Advanced experience in microbiology and molecular biology techniques, especially bacterial growth and cloning.
• Advanced experience in protocol development and optimization procedures.
• Demonstrated ability to serve as a technical resource, troubleshoot laboratory process problems and recommend process and database improvements.

Notes:  This is a 1 year term appointment with the possibility of renewal and with the possibility of conversion to career based upon satisfactory job performance, continuing availability of funds, and ongoing operational needs.  Selected candidate will be hired at the Research or Sr. Research Associate classification based on the candidate's level of experience and qualifications. Incumbents in these positions are represented by a union for collective bargaining purposes. Work will be performed at the DOE Joint Genome Institute (JGI) in Walnut Creek, CA. Job may require occasional travel, weekend, and after-hours work. Detailed information about the Joint Genome Institute program can be found on the World Wide Web at the following location:

About Us
Rapid technological advances in genomics have transformed modern biology. From its inception, the Department of Energy Joint Genome Institute has been at the forefront of large-scale sequence-based science. Learn more about JGI happenings at and
Lawrence Berkeley National Laboratory addresses the world’s most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Berkeley Lab’s science is a global enterprise. Our scientists work at the frontiers of knowledge to better understand our universe and to address the challenges facing our nation and our planet. Read about our excellent benefits (  

Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race,color, religion, sex, national origin, disability, age or protected veteran

How to Apply

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