This individual’s responsibilities will include, but are not limited to, execution of immune cell-based assays and various biochemical assays. These assays include primary immune cell bioactivity assays, cytokine / chemokine ELISAs, Taqman quantitative PCR, and flow cytometry, as well as (bio)analytical methods such as tissue processing / organic extraction and reverse phase HPLC. In addition, responsibilities will include test article preparation and sample management.
The level of independence in conducting the duties of this position will be determined based on the incumbent’s experience level.
Conduct experiments that include but are not limited to:
Preparation and characterization of various test articles for in vitro / in vivo experiments.
In vivo dosing of mice with test articles and subsequent tissue harvest.
Purification of immune cell populations from human blood and animal tissue, and medium through-put screening of immunomodulatory compounds.
ELISA analysis of cell culture supernatants and various biological fluids.
Isolation of RNA from cells and tissues and preparation of cDNA for quantitative PCR.
Tissue processing and HPLC analysis of analytes.
Analyze experimental data, prepare and submit detailed technical reports, and present results at project team and departmental meetings.
Day-to-day coordination of compound collections, laboratory reagents and maintenance of key equipment.
Work as a member of an integrated and matrixed project team in a highly collaborative work environment.
Document all scientific work in lab notebooks, follow established Dynavax standard operating procedures on data documentation and notebooks, and adhere to Dynavax Quality Systems standards.
Support the organization in maintaining a work environment focused on scientific integrity and quality, and one that fosters learning, open communication, collaboration, integration, and team work.
Other duties as assigned.
BS/MS in life science either in immunology, biochemistry, analytical chemistry and/or other related discipline.
Required experience will vary depending on level: RA I level is BS and at least 1+ year in biological sciences with focus in immunology, biochemistry and/or other related discipline; RA II is BS and 2-5 years with similar background, or an MS with 1+ year.
Relevant laboratory experience includes: aseptic cell culture of primary and established immune cell lines, ligand binding assays (e.g., ELISA, MSD, Luminex), FACS operation, quantitative PCR and HPLC.
Experience in and/or willingness to conduct experiments with laboratory mice, including dosing of mice with therapeutic compounds and assisting in surgical procedures / tissue extraction.
Ability to isolate and culture human immune cell subsets is highly desired.
Must be capable of working independently, detail-oriented, and must possess excellent record-keeping and strong organizational skills.
Proficiency with MS Word, Excel, Powerpoint and GraphPad Prizm.
Additional knowledge and skills desired, but not required:
Knowledge of immunology and analytical methods is highly preferred.
Basic knowledge of statistics is a plus.