The CGND is looking for a well-organized, methodical, and highly motivated Research Associate. This is an exciting opportunity to become part of a dynamic group that is investigating the interactions between motor neurons and glial cells in ALS pathology using hiPSC cell-based models combined with next generation sequencing and CRISPR technology. Our particular focus is to examine the transcriptional, translational, and functional impact of causative ALS mutations in a cell type specific manner. The individual will interface with the Director and key scientific staff to assume responsibility for hiPSC culture, differentiation, functional assays, and RNA harvesting for NGS. The Research Associate will be part of an interdisciplinary team working in a fast-paced environment.
Responsibilities include, but are not limited to:
- Conduct, design, and troubleshoot experiments, both independently and in collaboration with colleagues;
- CRISPR-based genome editing and transcriptional perturbations of causative ALS genes;
- Differentiation of hiPSCs into motor neurons and glial cells;
- Consult with scientific literature and other scientists as needed to better understand scientific experiments and problems, and propose solutions to emerging problems;
- Perform routine biochemical and molecular biology techniques (PCR, nucleic acid purification, qPCR, ELISA, Western blotting, etc.);
- Record and communicate findings accurately and professionally.