We are seeking scientists with knowledge of molecular biology and biochemistry to contribute to our efforts pertaining to fluorescent in situ sequencing (FISSEQ). You will work with cells and tissue to develop fluorescent molecular detection methods. Simultaneously, you will apply your understanding of molecular interactions between enzymes, tissue environment, hydrogels and surfaces to tackle challenges of molecule immobilization, extension, amplification and sequencing. The successful candidate will have demonstrable experience and the enthusiasm to work as a member of a multi-disciplinary team in a fast-paced, entrepreneurial, start-up environment.
Specific goals will include developing FISSEQ technology expand the capabilities of current methods and protocols. These responsibilities will include development and validation of experimental protocols, organization of participation in collaborative efforts, writing and assisting with peer-reviewed publications, patent applications, and grant proposals, biological experimentation in synthetic biology and sequencing contexts, and providing guidance to multiple interdisciplinary development teams.
- Develop and optimize methodologies for Fluorescent detection of molecules utilizing both in-vivo and in-vitro approaches;
- Responsible for developing and optimizing FISSEQ in tissue sections and cell culture;
- Develop protocols to facilitate rapid optimization of fixations, permeabilization and sample stability in cells and tissue;
- Standardize FISSEQ biochemistry with an emphasis on repeatability, process streamlining and cost efficiency;
- Conceptualize and implement technical advances related to the fixation, permeabilization, and stability of cells and tissue samples;
- Work with a cross-functional, collaborative team to adapt for commercialization;
- Analyze data and prepare comprehensive reports/presentations for fellow team members and management to show progress along project timelines;
- Contribute to the company’s intellectual property portfolio by documenting results and filing for IP protection.