A postdoctoral position is open at the Institute of Pathophysiology, First Faculty of Medicine, Charles University in Prague, Czech Republic for the project studying the role of extracellular microRNA in tumor biogenesis and dissemination.
Background: MicroRNAs are short non-coding RNAs (19-23nt) that negatively regulate gene expression by translational repression and/or mRNA of up to 60% of coding genes. Extracellular (circulating, secreted) microRNAs are an extracellular form of microRNAs which is in contrast to the longer species of RNA surprisingly stable and detectable in virtually all body fluids. It is well documented that tumor-associated microRNAs are released from the tumor into the circulation, where they exhibit extraordinary stability. However, the biological function of these circulating microRNAs in tumor biogenesis, reprogramming gene expression within target cells or intracellular communication remaining largely enigmatic. Any knowledge is lacking whether these microRNAs can enter the tissues, and whether they can aide in tumor progression and/or dissemination.
Project: In proposed study in vivo and in vitro assays answering the questions of a potential role of extracellular microRNAs in tumor biogenesis using mouse model and human hematological malignancies are being developed. The trafficking of microRNAs, their regulation and functionality in target tissues and distribution of their protein binding partners in body fluids are being characterized currently.
Methods: molecular cloning and mutagenesis, microRNA isolation and quantification, gene reporter assays, cell culturing and transfections, RNA sequencing, RIP and ChIP seq, WB, FACS, immunoassays and fluorescent microscopy, animal work.