Postdoctoral research fellowship - microRNA biology in carcinogenesis | GenomeWeb

Postdoctoral research fellowship - microRNA biology in carcinogenesis

Organization
First Faculty of Medicine, Charles University in Prague
Job Location
U Nemocnice 5
128 53 Prague
Czech Republic
Salary
2300 Eur/month brutto
Job Description

A postdoctoral position is open at the Institute of Pathophysiology, First Faculty of Medicine, Charles University in Prague, Czech Republic for the project studying the role of extracellular microRNA in tumor biogenesis and dissemination.

 Background: MicroRNAs are short non-coding RNAs (19-23nt) that negatively regulate gene expression by translational repression and/or mRNA of up to 60% of coding genes.  Extracellular (circulating, secreted) microRNAs are an extracellular form of microRNAs which is in contrast to the longer species of RNA surprisingly stable and detectable in virtually all body fluids. It is well documented that tumor-associated microRNAs are released from the tumor into the circulation, where they exhibit extraordinary stability. However, the biological function of these circulating microRNAs in tumor biogenesis, reprogramming gene expression within target cells or intracellular communication remaining largely enigmatic. Any knowledge is lacking whether these microRNAs can enter the tissues, and whether they can aide in tumor progression and/or dissemination.

Project: In proposed study in vivo and in vitro assays answering the questions of a potential role of extracellular microRNAs in tumor biogenesis using mouse model and human hematological malignancies are being developed. The trafficking of microRNAs, their regulation and functionality in target tissues and distribution of their protein binding partners in body fluids are being characterized currently.

Methods: molecular cloning and mutagenesis, microRNA isolation and quantification, gene reporter assays, cell culturing and transfections, RNA sequencing, RIP and ChIP seq, WB, FACS, immunoassays and fluorescent microscopy, animal work. 

Requirements

We seek for a motivated candidate (only foreign, PhD degree up to 10 years) capable of innovative thinking and independent work. Candidate should be familiar with cell and molecular biology methods. Bioinformatic data analysis skills are appreciated.

Duration: 2 years

Start: 1.1. 2016

Funding: 2300 Eur / month

How to Apply

Application should be send to Dr. Vitek Pospisil (vitek_pos@hotmail.com, T: 732 953372) including CV, list of publications and a cover letter outlining your research interests, a brief summary of your current work, and research skills.

Application deadline: 24th August 2015

 

For further details see:

http://www.cuni.cz/UKEN-318.html#2 ,  http://www.cuni.cz/UKEN-178.html#6 , 

and  http://patofyziologie.lf1.cuni.cz/microrna-and-transcription-factors

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