Postdoctoral Position in Molecular Biology and Technology Development in the Lee lab. The laboratory of Je H. Lee is seeking a postdoc fellow for multi-investigator research on profiling olfactory receptors in situ for spatial mapping of their connectivity using fluorescent in situ sequencing (FISSEQ; Lee et al., Science 2014). We would like to investigate how specific odor-responsive neurons and their projections are organized at the olfactory bulb using in situ genetic analysis, high resolution imaging, and optogenetic tools. In our approach, FISSEQ allows one to perform next generation sequencing directly within the tissue by converting tissue specimens into a sequencing flow cell. Our goal is to develop highly sensitive and specific as well as highly multiplexed OR mRNA detection methods in situ.
Beyond neuroscience, our method for profiling the transcriptome in situ in an unbiased manner using high resolution microscopy could be transformative for developmental, cell, and cancer biology. The Lee Lab is highly collaborative, and we work with various groups in the neuroscience and cancer programs at CSHL. CSHL is renowned for developing cutting-edge technologies in molecular biology and genetics and applying them to ask key questions in biology. Over 8,000 scientists come to various CSHL courses & meetings per year, offering unparalleled opportunities to interact, exchange ideas, and network with world-class scientists across the globe.