Postdoctoral Position in Cancer Biology in the Lee lab at Cold Spring Harbor Laboratory
The spatial complexity of metabolic and epigenetic reprogramming and its role in cancer cellular evolution.
The laboratory of Je H. Lee is seeking a postdoctoral fellow for research on molecular and cellular evolution of single cells in mouse and human cancers, using fluorescent in situ sequencing (FISSEQ), a novel imaging platform for high-throughput RNA sequencing in situ for direct visualization of transcripts in intact tissues (Lee et al. Science 2014). We are also developing targeted RNA-seq and epigenetic in situ sequencing methods for discriminating altered methylation patterns in various tissue types and structures. We are looking for candidates with an interest in metabolism, epigenetic noise, microenvironment, and cancer cell evolution in solid tumors. We welcome candidates with experience and/or interest in animal models, genetics, computational modeling, single cell analysis, high-throughput sequencing, cell signaling, and/or technology development.
While single cell variations due to genetic and epigenetic differences are important, the micro-environmental heterogeneity also plays a major role in cancer cell evolution in vivo. Our technology could allow us to identify biomarkers of metabolic reprogramming, EMT, dissemination, and metastasis as well as the stromal tissue adjacent to the tumor across various stages in situ in vivo. We are also investigating whether the cellular plasticity and fitness correlate with their microenvironment as well as the tumor metabolism, type, size, and location—-poorly understood, yet key indicators of clinical prognosis. We will utilize both genetically engineered mouse models and human patient samples from collaborating medical centers. Our ultimate goal is to demonstrate a novel paradigm in tumor pathology and biomarker discovery, in which image pixels in next generation sequencing technology are generated directly on clinical tissue specimens, preserving the biological context of genetic alternation and gene expression. This project is a collaborative effort at Cold Spring Harbor Laboratory, utilizing genetically engineered mouse and organoid models (Tuveson Lab), in vivo imaging (Egeblad Lab), single cell sequencing (Wigler Lab), cancer immunology (Fearon Lab), and non-coding RNA (Spector Lab).