Postdoctoral Fellow (Synthetic Biology/Strain Engineering) - 80127
The Department of Energy Joint Genome Institute - DOE JGI (a division of the Lawrence Berkeley National Laboratory) has an exciting opportunity available for a postdoctoral fellow. As part of a large multidisciplinary research program at JGI, we seek to understand the functional genomics of microbial metabolism to help enable the development of biofuels, understand carbon cycling and the impacts of climate change. This position will play an important role in domesticating various non-conventional bacterial strains to be used for expression of natural product pathways and other fields of research in Synthetic Biology to support the JGI through collaborative projects. This includes development of molecular tools and methodologies to integrate, replace, delete, and mutate genes of interests efficiently and in high-throughput manner. This position directly reports to the Synthetic Biology - Pathway Engineering group lead.
• Develop tools and methodologies to domesticate and facilitate engineering of non-conventional bacterial species. The specific job duties include:
• Establish optimal culture conditions
• Establish efficient protocols for transformation
• Develop and construct plasmid systems
• Analyze comparative expression profiles (qPCR and RNA sequencing) in several different culture conditions and create promoter library
• Develop molecular tools and procedures efficiently insert, replace, delete, and mutate targeted locus in a chromosome