A postdoctoral position is currently available, in the laboratory of Dr. David L. Spector, for a highly motivated outstanding individual to study Random Autosomal Monoallelic Gene Expression, a process in which transcription occurs stochastically from only one of two homologous alleles in a diploid cell, independent of the genetic sequence or parental origin. Recently, we found that upon differentiation of mouse embryonic stem cellls to neural progenitor cells, random autosomal monoallelic expression increases from 0.5% to 3.0%, and 8% of these genes exhibit transcriptional compensation. A complete understanding of this process will have significant implications when considering the genetic basis of both Mendelian and complex disorders as well as pathologies associated with haploinsufficiencies.
This project will employ state-of-the art technology to provide fundamental advances into characterizing the relationship of random autosomal monoallelic expression to various differentiation paradigms and will define how it is established, maintained, and regulated. Experience in mouse embryonic stem cell culturing and differentiation, genomic analysis, molecular biology, and mouse models is a plus.
Cold Spring Harbor Laboratory provides a highly dynamic and interactive environment for scientific research. In addition, our Meetings and Courses program provides an opportunity for interacting with a broad range of researchers and exposure to timely advances in many areas of scientific research.