The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. (HJF) is seeking a Postdoctoral (210517) for the Gynecologic Cancer Translation Research Center of Excellence (GYN-COE) Program located at the Women’s Health Integrated Research Center at Inova Health System in Fairfax, Virginia to contribute to gynecologic cancer biomarker research using advanced techniques in mass spectrometry and quantitative proteomics. HJF provides scientific, technical and programmatic support services to GYN-COE.
The projects are specifically aimed at development and application high resolution mass spectrometric techniques for differential identification of gynecologic cancer biomarkers from tissue biopsies, proximal fluids and serum collected from cancers of the uterus, ovary and fallopian tube using stable isotope labeled and label-free quantitative proteomics, including the use of data-dependent and data-independent acquisition techniques. The projects will involve technical developments in mass spectrometry toward improved analytical precision and throughput for assay of these clinical samples using mass spectrometry.
1. Sample preparation for proteomics to include extraction of proteins from cells, serum protein depletion, protein separation, digestion and desalting.
2. Operation of nanoflow high performance liquid chromatographs.
3. Operation of high resolution mass spectrometers.
4. Development and standardization of high throughput proteomic workflows.
5. Proteomic data analysis and interpretation.
6. Validation of proteomic results by biochemical methods.
7. Preparation of scientific reports and manuscripts.
8. Presentation of scientific results at GYN-COE group meetings and national conferences.